重组质粒pEgr-P１6在Ec9706细胞中的辐射诱导凋亡作用

doi:10.3969/j.issn.1004-616x.2004.03.001

Carcinogenesis, Teratogenesis & Mutagenesis ›› 2004, Vol. 16 ›› Issue (3): 129-131.doi: 10.3969/j.issn.1004-616x.2004.03.001

The Apoptosis Effect of pEgr-P１6 Recombined Plasmid in EC9706 cell Induced by Ionizing Irradiation

WU Cong-mei，HUANG Tian-Hua;XIE Qing-Dong，et al

Research center of Reproductive Medicine,Shantou University Medical college,Shantou 5１504１,china

Received:2004-02-10 Revised:2004-03-16 Online:2004-05-30 Published:2004-05-30
Contact: HUANG Tian-Hua

Abstract

Abstract: BACKGROUND ＆ AIM： To investigate the effect on apoptosis of the recombined plasmid pEgr-P１6 induced by ionizing irradiation in esophageal squarous carcinoma EC9706 cell line． MATERIAL AND METHODS： The recombined plasmid pEgr-P１6 was transfected into EC9706 cell with lipofectamine． Quantitative RT-PCR was performed to study the expression of P１6 in EC9706 and the apoptosis of Ec9706 cells after transfection induced by ionizing irradiation． RESULTS：The＇was cwve valefionshp between and time during 2～24 h after irradiation and thereafter decreased gradually, in contrast to negative P１6 expression in the control． The induction of apoptosis in P１6 gene therapy plus irradiation group was higher than that in either plasmid or irradiation group alone． CONCLUSION：γ-ray can induce expression of pEgr-P１6 plasmid in Ec9706 cells and enhance the apoptosis of cancer cells．These results may establish important experimental basis for gene-radiotherapy of esophageal carcinoma．

Key words: Egr-１ promoter, γ-ray, P１6 expression, apoptosis

WU Cong-mei, HUANG Tian-Hua, XIE Qing-Dong, et al. The Apoptosis Effect of pEgr-P１6 Recombined Plasmid in EC9706 cell Induced by Ionizing Irradiation[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2004, 16(3): 129-131.

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The Apoptosis Effect of pEgr-P１6 Recombined Plasmid in EC9706 cell Induced by Ionizing Irradiation

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